Other

Part:BBa_K4374011:Design

Designed by: Sarah García Hualde   Group: iGEM22_Navarra_BG   (2022-08-26)


PEPC1 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 858
    Illegal BamHI site found at 873
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 24


Design Notes

This part supports 3A Assembly method. A mutation in position 142 was generated to eliminate EcoRI site (G into C).



Source

GenBank At1g53310


References